Album was composed by and was released on May 21, 2008. Soundtrack consists of tracks with duration over about 60 minutes. Album was released by Team Entertainment.
CD 1
| 1 | Twenty-Four Warriors (Character Select Screen) | 01:19 |  |
| 2 | To the Next Land (Map Display Screen) | 02:44 | |
| 3 | Fair and Square! (Opponent Display Screen) | 00:08 | |
| 4 | The Remote Yagyu Village (Bamboo Thicket Stage) | 01:46 | |
| 5 | Isle of the Dead-Time of the Horse (Isle of the Dead Stage-Day) | 02:32 | |
| 6 | Isle of the Dead-Time of the Dog (Isle of the Dead Stage-Night) | 02:26 | |
| 7 | Return of the Viking (Harbor Stage) | 02:24 | |
| 8 | In Front of Kokain Gate (Kokain Stage) | 01:33 | |
| 9 | Kamuy-Kotan: Kokujo Waterfall (Nakiyama Stage) | 01:46 | |
| 10 | Noshinjin Shrine: Offering Scene (Festival Stage) | 01:15 | |
| 11 | Sanpougatake-Time of the Dog (Silver Grass Stage-Night) | 02:07 | |
| 12 | Sanpougatake-Time of the Monkey (Silver Grass Stage-Evening) | 02:40 | |
| 13 | Desert Town-Central Market (Arabian Market Stage) | 01:19 | |
| 14 | Sunset Gunman (American West Stage-Evening) | 03:19 | |
| 15 | Town in the Wastelands (American West Stage-Noon) | 01:40 | |
| 16 | Genkoku Temple: Lord Hua Long (Chinese Temple Stage) | 01:01 | |
| 17 | Castle of Despair in the Light (Western Castle Stage-Day) | 02:27 | |
| 18 | Castle of Despair in the Darkness (Western Castle Stage-Night) | 02:05 | |
| 19 | House of the Red Devil (English Cabin Stage) | 02:30 | |
| 20 | The Silver Bull Tavern (Spain Barroom Stage) | 02:10 | |
| 21 | Stand and Fight (Last Boss-War Room Stage) | 02:01 | |
| 22 | Tenkou, The Snowy Owl Castle-Time of the Serpent (Japanese Castle Stage-Day) | 02:43 | |
| 23 | Tenkou, The Snowy Owl Castle-Time of the Hog (Japanese Castle Stage-Night) | 02:23 | |
| 24 | Hei'an Highway-Fortress of Chaos (South Korea Stage) | 00:47 | |
| 25 | Victory Screen | 00:07 | |
| 26 | Game Over | 00:10 | |
| 27 | Request for a Rematch (Continue Screen) | 01:20 | |
| 28 | Prologue (Character Introduction) | 02:50 | |
| 29 | After the Battle... (Ending) | 04:21 | |
| 30 | Another Feast (Ending Credits) | 02:19 | |
