Album was composed by William Ross and was released on December 16, 2008. Soundtrack consists of 26 tracks tracks with duration over about 60 minutes. Album was released by Back Lot Music, A Division of Universal Studios, Inc..
CD 1
| 1 | SoupDave Stewart, Glen Ballard & Gary Ross | 01:43 |  |
| 2 | It's Great to Be a RatDave Stewart, Gary Ross & Glen Ballard | 01:23 | |
| 3 | Main Title / PrologueWilliam Ross | 02:38 | |
| 4 | The Village of DorWilliam Ross | 02:17 | |
| 5 | Andre & BoldoWilliam Ross | 01:27 | |
| 6 | The Soup Is ServedWilliam Ross | 01:11 | |
| 7 | Roscuro's FallWilliam Ross | 02:42 | |
| 8 | A King's SadnessWilliam Ross | 02:00 | |
| 9 | Mouse World / A Mouse Is BornWilliam Ross | 03:11 | |
| 10 | Lonely RoscuroWilliam Ross | 01:15 | |
| 11 | The Royal LibraryWilliam Ross | 01:30 | |
| 12 | Once Upon a TimeWilliam Ross | 02:30 | |
| 13 | I Am a Gentleman / Mig's StoryWilliam Ross | 03:39 | |
| 14 | BanishmentWilliam Ross | 03:08 | |
| 15 | In the DungeonWilliam Ross | 01:02 | |
| 16 | Cat and MouseWilliam Ross | 02:03 | |
| 17 | Roscuro and DespereauxWilliam Ross | 02:11 | |
| 18 | Mig Steals the CrownWilliam Ross | 01:20 | |
| 19 | Roscuro's ApologyWilliam Ross | 03:45 | |
| 20 | Gregory Gives Mig AwayWilliam Ross | 01:02 | |
| 21 | The QuestWilliam Ross | 03:56 | |
| 22 | Despereaux Is BackWilliam Ross | 03:15 | |
| 23 | Boldo and Despereaux Charge!William Ross | 01:39 | |
| 24 | A Change of HeartWilliam Ross | 02:14 | |
| 25 | Rescuing the PrincessWilliam Ross | 03:07 | |
| 26 | EpilogueWilliam Ross | 02:43 | |
